Monoclonal Antibody based Blocking ELISA for Diagnosis of Brucellosis

Background: Porcine brucellosis is a contagious and emerging zoonosis but neglected in most of the endemic countries including India. The disease pigs rarely reported due to non-availability diagnostics or major focus on bovine brucellosis. Hence, necessity was felt diagnose porcine by Monoclonal antibody based ELISA, RBPT STATS for detection anti-Brucella antibodies record spatial seroprevalence country. Methods: Rose Bengal Plate Test (RBPT), Standard tube agglutination test (STAT) Bru Alert monoclonal c-ELISA employed screen 148 sera samples presence Brucella antibody. Result: A total were collected tested, overall prevalence infection pig 43.2% per cent (64/148) C-ELISA followed 40% (60/148) STAT 25% (40/148) study area comparatively high seropositivity found female animals. agreement between two excellent (Kappa = 0.167) also, chisquare indicated an evidence strong diagnostic weapon swine